Reference Data Summary

The reference data used by ITSoneWB are listed here. Please refere to tools documentation to use them.

ITSoneDB vs. shotgun mapping tool

ITSoneDB (r131) BioMaS indexes can be downloaded here.

BioMaS

ITSoneDB (r138) BioMaS indexes can be downloaded here.

Mopo16S

Mopo16S reference primer pair can be downloaded here.

Prepare primer input inference

The reference data files are hosted on GitHub and can be easily downloaded:

1. ITS1_r131_plus_flanking_region.fna.gz.

2. node2tax_name_path.tsv.gz.

Barcoding Gap

The Distances files can be downloaded here.

Qiime

Qiime ITSoneDB reference data are available here.

On Galaxy, add ITSoneDB_r138_NR99.fasta file to qiime_rep_set.loc loc file.

Qiime 2

Qiime 2 ITSoneDB reference data are available here.

On Galaxy, add ITSoneDB_r138_NR99.taxa.qza file to qiime2_taxonomy.loc loc file.

Mothur

Mothur ITSoneDB reference data are available here.

On Galaxy:

1. Add the ITSoneDB_r138_NR99_taxa.tsv file to mothur_aligndb.loc loc file.

2. Add the ITSoneDB_r138_NR99_taxa.tsv file to mothur_taxonomy.loc loc file.