Welcome to itsonewb’s documentation!¶
The amplicon-based metagenomic analysis (i.e. DNA metabarcoding), represents a powerful tool to rapidly, efficiently and cheaply investigate large microbial communities. The growing interest in its application results in a concomitant increase of appropriate resources designed to manage and analyze data in FAIR-compliant way (Wilkinson et al., 2019).
In this context, ITSoneWB (ITSone WorkBench) represents a well-defined environment collecting and integrating the high quality ITS1 reference collection in ITSoneDB (Santamaria et al., 2017) with DNA metabarcoding well-established analysis pipelines and new tools in an easy-to-use service addressing the eukaryotic domain of life. Specifically, BioMaS, QIIME (Caporaso et al., 2010), QIIME2 (Bolyen et al., 2019) Mothur (Schloss et al., 2009) pipelines were included and new tools to infer the ITS1 Barcoding gap and PCR primers pair in a user defined taxonomic range and to identify ITS1 regions in shotgun metagenomic data were designed, developed and deployed in a Galaxy environment.
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For any issue related to ITSoneWB please contact us by mail.
General Information